Titlebook: Methods of Protein Microcharacterization; A Practical Handbook John E. Shively Book 1986 Springer Science+Business Media LLC 1986 amino aci

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Amino Acid Analysis With Postcolumn Fluorescent Derivatizationbut also for confirming the results from other types of analyses. Most current commercial analyzers are essentially modernized versions of the original autoanalyzer of Spackman, Stein, and Moore (.), designed some 30 yr ago. However, other methodologies for amino acid analysis are now becoming more

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Comparison of the Spinning Cup and Gas-Phase Instrumentscer became the workhorse of protein chemists. The nominal operating range of the Beckman instrument was 100–500 nmol of protein. With the exception of a few minor changes, the Beckman instrument has remained largely unchanged over a period of 15 yr. Major changes in the reagent/solvent delivery syst

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Gas-Phase Protein/Peptide Sequenceructure of proteins and peptides (.). This chemical process is a repetitive reaction in which the alpha-amino group of the polypeptide chain is coupled to phenylisothiocyanate (PITC) under alkaline conditions, whereupon the terminal amino acid is cleaved from the remainder of the peptide with acid (.

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Design of a Multipurpose Sequencerthe effectiveness of the sequential degradations, increased the repetitive yields, lowered the background contaminations of the released amino acid derivatives, and allowed considerably extended degradation of polypeptides in comparison to any of the manual techniques available at that time. This so

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