Titlebook: Methods in Human Cytogenetics; H. G. Schwarzacher,U. Wolf,E. Passarge Book 1974 Springer-Verlag Berlin Heidelberg 1974 Chromosom.Cytogenet

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https://doi.org/10.1007/978-3-642-65787-0Chromosom; Cytogenetics; Zytogenetik; cell; diagnosis; genetics; translation

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Preparation of Metaphase Chromosomes,Chromosome preparations can principally be obtained from all tissues and suspensions in which mitoses occur. The initiation of cell cultures particularly suitable for chromosomal analysis owing to abundant mitoses, has been described in Chapters I and II. Tissues obtained directly from biopsies are also suitable for chromosome preparations.

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The Diagnosis of X-Chromatin by the Leukocyte Test,The morphology of . in peripheral . differs from that of other tissues. The so-called leukocyte test for the diagnosis of X-chromatin is based on the identification of specific nuclear formations which were first described and recognized as important by Davidson and Smith in 1954.

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978-3-642-65789-4Springer-Verlag Berlin Heidelberg 1974

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Cell Cultures from Tissue Explants,l) are taken from the body, reduced to small pieces, and set up in vitro in a nutrient medium. There are a number of methods available to prevent expiant fragments from floating in the nutrient medium. In these socalled primary cultures cells divide which are usually morphologically heterogeneous. D

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Culture and Preparation of Cells from Amniotic Fluid,2; Milunsky, 1973; Nadler, 1972), owing to the frequency of chromosomal aberrations, nearly all of which can be recognized. Prenatal cytogenetic diagnosis is particularly indicated in the following cases:

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