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Titlebook: Methods in Cellular Imaging; Ammasi Periasamy (Director, Professor of Biology a Book 2001 American Physiological Society 2001 Cellular Ima

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發(fā)表于 2025-3-21 17:39:09 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Methods in Cellular Imaging
編輯Ammasi Periasamy (Director, Professor of Biology a
視頻videohttp://file.papertrans.cn/633/632279/632279.mp4
概述Well illustrated book explains basic concepts and imaging procedures.Authors descrube approaches to selecting epiflourescence microscopy, detectors, and image acquisition and processing software for d
叢書名稱Methods in Physiology
圖書封面Titlebook: Methods in Cellular Imaging;  Ammasi Periasamy (Director, Professor of Biology a Book 2001 American Physiological Society 2001 Cellular Ima
描述Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The aut
出版日期Book 2001
關(guān)鍵詞Cellular Imaging; biology; biophysics; cell; cell biology; gene; gene expression; membrane; microscopy; prote
版次1
doihttps://doi.org/10.1007/978-1-4614-7513-2
isbn_ebook978-1-4614-7513-2Series ISSN 2628-7471 Series E-ISSN 2628-748X
issn_series 2628-7471
copyrightAmerican Physiological Society 2001
The information of publication is updating

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Detectors for Fluorescence Microscopytical components in fluorescence microscopy is the imaging device because it determines whether the fluorescence may be detected, the relevant structures resolved, or the dynamics of a process visualized (Aikens et al., 1988; Becker, 1996; Bookman, 1990; Bright and Taylor, 1986; Herman, 1998; Perias
地板
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Basics of a Light Microscopy Imaging System and Its Application in Biologyernike, 1958), differential interference contrast (Allen et al., 1969), epifluorescence (Ploem, 1967), laser scanning confocal (Minsky, 1957), and now multiphoton confocal microscopy (Denk et al., 1990). As the imaging technology evolves (Inoué and Spring, 1997), the biologist works to prepare fixed
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Laser Scanning Confocal Microscopy Applied to Living Cells and Tissuesitude and time course of individual cellular changes. Responses within single cells may also show spatial heterogeneity. For these reasons, three-dimensionally resolved approaches are needed to study individual cells as they respond to imposed stimuli and stresses. Increasingly, confocal microscopy
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Basic Principles of Multiphoton Excitation Microscopy G?ppertMayer in 1931. Generating three-dimensionally resolved microscopic images based on nonlinear optical excitation was postulated in the 1970s (Gannaway and Sheppard, 1978; Wilson and Sheppard, 1984). The definitive experiment was done by Denk, Webb, and co-workers (1990), who accomplished two-
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Building a Two-Photon Microscope Using a Laser Scanning Confocal Architecture al., 1990), two-photon excitation (TPE) fluorescence microscopy can be considered a comparatively young technique in far-field fluorescence optical microscopy. This technique has advantages over both widefield and confocal laser scanning microscopy (Wilson and Sheppard, 1984; Wilson, 1990; Pawley,
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