Titlebook: Methods in Biological Oxidative Stress; Kenneth Hensley,Robert A. Floyd Book 2003 Humana Press 2003

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Book 2003-effects, xenobiotic toxicity, carcinogenesis, and the aging process, to name just a few. Because of the centrality of oxidative processes to normal and abnormal tissue function, it has become imperative to develop appropriate analytical techniques to facilitate the quantitation of significant react

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1557-2153 luding neurodegenerative disease, drug side-effects, xenobiotic toxicity, carcinogenesis, and the aging process, to name just a few. Because of the centrality of oxidative processes to normal and abnormal tissue function, it has become imperative to develop appropriate analytical techniques to facil

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Measurement of Isoketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Massn, e.g., 4-hydroxynonenal, by orders of magnitude (.). Adduction of proteins frequently leads to altered protein function (.–.). This in turn can lead to cellular dysfunction, which may be causally linked to the pathogenesis of disease processes.

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Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring ansitivity for single-strand breaks (.,.). This simple assay has come into widespread use because of its sensitivity, need for few cells, applicability to nearly all eukaryotic cells, and ease in processing numerous samples (.).

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Analysis of Aldehydic Markers of Lipid Peroxidation in Biological Tissues by HPLC with Fluorescence ldehydic by-products, including the use of ultraviolet (UV)-Vis spectrometry to measure the heat mediated-condensation products of aldehydes with thiobarbituric acid in the thiobarbituric acid-reactive substances (TBARs) assay. Although this method will provide an overall measure of aldehyde levels,

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