Titlebook: Metabolic Signaling; Methods and Protocol Sarah-Maria Fendt,Sophia Y. Lunt Book 2019 Springer Science+Business Media, LLC, part of Springer

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A Protocol to Compare Methods for Untargeted Metabolomics,d and optimized for a small number of select metabolites, very few have been assessed on the basis of global metabolite coverage. Thus, when performing untargeted metabolomics, researchers often question which combination of extraction techniques, chromatographic separations, and mass spectrometers

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Measuring the Nutrient Metabolism of Adherent Cells in Culture,using techniques such as liquid chromatography-mass spectrometry (LC-MS). This chapter describes in detail a method for metabolite extraction from cultured adherent mammalian cells to collect both polar and nonpolar intracellular metabolites. This chapter also describes experimental design considera

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Determining Compartment-Specific Metabolic Fluxes,cells. Mitochondria of adherent cell cultures are made accessible by the addition of digitonin—a compound that selectively permeabilizes the cytosolic membrane without affecting mitochondrial integrity. The generated in situ mitochondria are subsequently used in a stable isotope labeling assay in wh