Titlebook: Metabolic Reprogramming; Methods and Protocol Salvatore Papa,Concetta Bubici Book 2023 The Editor(s) (if applicable) and The Author(s), und

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Raquel Saborano,Emma Shepherd,Ulrich L. Günther,Patricia F. Lalor

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1064-3745 ation advice from the experts.This volume provides state-of-the-art methods and protocols from researchers in the field of metabolism. Chapters guide readers through methodologies measuring different aspects of lipid metabolism, quantifying the amino acids, mitochondrial and intracellular by-product

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Real-Time Monitoring of Hydrogen Peroxide Levels in Yeast and Mammalian Cells,developed over the last few years that enable such non-disruptive monitoring with high spatiotemporal resolution. In this chapter, we describe the use of these genetically encoded sensors to directly monitor H.O. dynamics in yeast and cultured mammalian cells.