Titlebook: Metabolic Reprogramming; Methods and Protocol Salvatore Papa,Concetta Bubici Book 2023 The Editor(s) (if applicable) and The Author(s), und

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Methods for Monitoring Mitochondrial Biogenesis and Turnover in Cultured Hepatocytes and Mouse Liveoscopy analysis revealed that majority of mitochondria were spatially heterogeneous with proteins from simultaneous new synthesis, maturation, and turnover in hepatocytes. MitoTimer reporter assay can specifically target to mitochondria and be used to monitor mitochondrial biogenesis and maturation

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Measuring the Oxidation State and Enzymatic Activity of Glyceraldehyde Phosphate Dehydrogenase (GAPce decreased GAPDH activity results in the rerouting of carbon flux away from glycolysis and toward the pentose phosphate pathway to produce the key cellular reductant and antioxidant, NADPH. Thus, the ability to probe GAPDH oxidation and activity provides an important snapshot of the intracellular

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Marta Iborra Pernichi,Jonathan Ruiz García,Nuria Martínez-Martín

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María Eugenia Monge,Manuela R. Martinefski,Mariela Bollini,Lucas B. Pontel