Titlebook: Metabolic Flux Analysis in Eukaryotic Cells; Methods and Protocol Deepak Nagrath Book 2020 Springer Science+Business Media, LLC, part of Sp

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Deepak NagrathIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Determination of Isotopologue and Tandem Mass Isotopologue Ratios Using Gas Chromatography Chemicalof assessing absolute intracellular metabolic fluxes. Resulting from feeding the organism of interest with a specifically isotope-labeled substrate, the principal characteristics of these labeling experiments are the metabolites’ non-naturally distributed isotopologue patterns. For the purpose of in

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Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C). Liquid chromatography coupled with mass spectrometry (LC/MS) has emerged as a frontrunner technique that combines two orthogonal separation strategies. While metabolomics requires separation of monoisotopic peaks, .C-MFA imposes additional demands for chromatographic separation as isotopologs of

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Deuterium Tracing to Interrogate Compartment-Specific NAD(P)H Metabolism in Cultured Mammalian Cellfer between molecules and organelles is commonly facilitated by pyridine nucleotides such as nicotinamide adenine dinucleotide phosphate (NADPH) and nicotinamide adenine dinucleotide (NADH). While often taken for granted, these metabolic reactions are critically important for maintaining redox homeo

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