Titlebook: Membrane Trafficking; Methods and Protocol Jingshi Shen Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

DRAFT

Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargohe Golgi. When traffic resumes upon temperature block release, kinetics of transport can be assessed by confocal microscopy through colocalization of the cargo with cis- and trans-Golgi markers. This method allows for testing various modes of intra-Golgi transports and can be adapted to investigate other steps of the secretory pathway.

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1064-3745 expertsThis detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to .in vitro. biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, whi

ANT

Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention Systemctive Hook (RUSH) system, a highly specific and sensitive tracking system with a high spatiotemporal resolution. Taking GLUT4 and GLUT1 as examples of unconventionally and conventionally secreted cargo respectively, we describe the steps to clone the cargoes in the RUSH vector and follow and quantif

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Live-Cell Superresolution Imaging of Retrograde Axonal Trafficking Using Pulse–Chase Labeling in Culailable microfluidic device to enable the labeling and tracking of retrograde axonal carriers, including (1) how to culture and transfect rat hippocampal neurons in the microfluidic device; (2) how to perform pulse–chase to label specific populations of retrograde axonal carriers; and (3) how to con

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Book 2022nown pitfalls.?.Authoritative and practical, .Membrane Trafficking: Methods and Protocols. provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs..

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