Titlebook: Melanoma; Methods and Protocol Kristian M. Hargadon Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Human mela

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外面

A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma CellsMelanoma cells have high glycolytic capacity. Glucose uptake is a key rate-limiting step in glucose utilization. Here we describe a simple protocol for measuring direct glucose uptake in living melanoma cells by flow cytometry.

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Model Systems for the Study of Malignant Melanoma,rstand melanoma biology for therapeutics development began with the employment of isolated cancer cells grown in cell cultures. However, these models lack in vivo interactions with the tumor microenvironment. Melanoma cell line transplantation into suitable animals such as mice has been informative

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Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editingt the cellular and molecular levels. Despite the impact that such advances have made on the clinical management of this cancer over the last decade, additional insights into factors that promote melanoma progression and therapeutic resistance are needed to combat this disease. CRISPR-Cas9 gene editi

LUCY

A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrixy to other types of tumor cells, form invadopodia, membranous invaginations rich in filamentous actin and several other proteins including matrix metalloproteinases (MMPs). MMPs degrade ECM structural proteins such as collagens, fibronectin, or laminin. Here we describe an assay that allows the?dete

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Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Techn patients, and it is important to understand these differences since they potentially can affect therapy response. Here we discuss a method of processing and assaying metabolism from direct ex vivo murine and human tumor samples using seahorse extracellular flux analysis. This provides real-time pr