Titlebook: Mass Spectrometry in Biomolecular Sciences; Richard M. Caprioli,Antonio Malorni,Giovanni Sindo Book 1996 Kluwer Academic Publishers 1996 A

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Richard M. Caprioli,Antonio Malorni,Giovanni Sindo

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Matrix-Assisted Laser Desorption Mass Spectrometry on a Magnetic Sector Instrument Fitted With an Arating focal plane array detector. High sensitivity (low fmol detection) was obtained with peptides such as renin substrate. Underivatised complex oligosaccharides were also detectable at the 200 fmol level. The advantages of the array detector for improved resolution (5000 FWHM) and the benefits in

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Electrospray Ionization Mass Spectrometry: Protein Structurear weight. In conjunction with peptide mapping and tandem MS, it has been used to elucidate the primary structure of peptides and proteins. ESI MS analysis alone is not sufficient for examination of secondary structure, but comparison of deuterium exchange experiments with information gained by othe

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Applications of Quantum Chemistry in Mass Spectrometry given on the semiempirical and . theoretical methods, their advantages and limitations. Beside energetics, various other parameters (geometry, bond order, energy partitioning, spin and charge density, free valence) can be used to characterise ion structures, reactions and possibly to predict fragme

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The Detection of High Mass-to-Charge Biological Ions by Fourier Transform Mass Spectrometry: Issues r of 150000, FTMS is seldom employed as a tool for the characterization of biological molecules. This is because the superior detection performance that is associated with FTMS rapidly degrades for ions with mass-to-charge ratios greater than 5000. The purpose of this chapter is to introduce the rea

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Quadrupole Mass Filters, Quadrupole Ion Traps and Fourier Transform Ion Cyclotron Resonance Spectromrap was mainly due to the Pauli’s group. Quadrupoles are widely employed in routine mass spectrometric analysis as well as in sophisticated applications. The principle of operation of mass filters and ion traps will be discussed. A short description of the ICR cell will be also presented