Titlebook: Mass Spectrometry Imaging of Small Molecules; Methods and Protocol Young-Jin Lee Book 2022 The Editor(s) (if applicable) and The Author(s),

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Metal-Assisted Laser Desorption Ionization Imaging Mass Spectrometryrol, free fatty acids, and triglycerides. In this chapter, we demonstrate the potential of metal-assisted LDI IMS through the analysis of spinal cord and kidney thin tissue sections after silver and gold metal deposition. We also detail typical step-by-step workflows and discuss the strength of the methods.

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Imaging of Plant Hormones with Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry. Here, we illustrate a technology for multiple-hormone imaging using Nano-PALDI MSI and discuss its potential in investigating the role of hormone signaling in plant development and stress responses.

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Gas-Phase Ion–Ion Reactions for Lipid Identification in Biological Tissue Sectionsdure is described here for the identification of phosphatidylcholine (PC) analyte cations using 1,4-phenylenedipropionic acid reagent dianions, it can readily be generalized to perform a diverse array of ion/ion reaction chemistries.

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Book 2022ng imaging, software,? data analysis, new instrumentation, and new methodological approaches. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily

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Extraction and Upconcentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass microstructured PDMS stamp is utilized to precisely control the matrix deposition area and the analyte extraction area. We describe here simple steps—including stamp fabrication, matrix application, and data-acquisition guideline—for the quantitative analysis of adsorbed peptides on hydrophobic surfaces.

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Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50?μm spatial resolution for a next-generation histopathological assessment of glycogen.

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