Titlebook: Mammalian Synthetic Systems; Francesca Ceroni,Karen Polizzi Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive lic

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Anti-CRISPR Proteins and Their Application to Control CRISPR Effectors in Mammalian Systems,robial innate immune system, which emerged from the evolutionary arms race between microbes and phages. This coevolution has also given rise to so-called anti-CRISPR (Acr) proteins that counteract the CRISPR-Cas adaptive immunity. Acrs constitutively block cognate CRISPR-Cas effectors, e.g., by inte

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Imaging ,-Adenosyl Methionine Dynamics in Living Cells Using an RNA-Based Fluorescent Sensor, Understanding the spatiotemporal dynamics of SAM within living cells is essential for deciphering its roles in maintaining cell homeostasis and in disease development. Here, we describe a protocol based on a recently reported SAM sensor exploiting a fluorogenic RNA and an RNA three-way junction for

canonical

High-Throughput Spectroscopic Analysis of mRNA Capping Level, translation initiation, and stability. Here, we describe a novel biosensor method designed to detect the presence of both cap structures and polyadenylation sites on mRNA molecules. This novel biosensor is sensitive to mRNA degradation and can quantitatively determine capping levels of mRNA molecul

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In Vitro Generation of Megakaryocytes from Engineered Mouse Embryonic Stem Cells,hat can provide insights into the design principles governing cell fate control. We are interested in reprogramming pluripotent stem cells with synthetic gene circuits to drive mouse embryonic stem cells (mESCs) down the hematopoietic lineage for the production of megakaryocytes, the progenitor cell

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https://doi.org/10.1007/978-1-0716-3718-0Cloning toolkits; Gene expression control; Synthetic epigenetics; CRISPR-based systems; Bioprocessing ap