Titlebook: Mammalian Synthetic Promoters; David Gould Book 2017 Springer Science+Business Media LLC 2017 synthetic promoters.gene therapy.pharmacolog

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Bioinformatically Informed Design of Synthetic Mammalian Promotersccur in identified gene modules, and the length of identified .-regulatory regions. Our method allows for the generation of synthetic promoter libraries with increased level of specificity and facilitates the selection of promoters that are highly active only under predefined gene expression profile

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Initial Considerations Before Designing a Promoter Construct,short-term promoter activity from an episomal vector, or does the promoter eventually need to be permanently active and be integrated into the genome or perhaps even to function in vivo? The final application will have a bearing on promoter design and vector of choice from the start of the study. In

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Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assayter. Electrophoretic mobility shift assay is a powerful approach to demonstrate the specific DNA sequence that is bound by a transcription factor and also to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demon

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Secreted Reporters for Monitoring Multiple Promoter Functioned medium of cultured cells or body fluids such as blood and urine, ex vivo. In this chapter, we will explore established secreted reporters and their applications and limitations for monitoring of promoter function. We will also describe both cell-based and blood-based assays for detecting three co

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Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivolar biology. Luciferase catalyzes an enzymatic reaction, which in the presence of the substrate luciferin produces photons of light relative to its molar concentration. The luciferase transgene can be genetically inserted at the first intron of a target gene to act as a surrogate for the gene’s endo

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Monitoring Promoter Activity by Flow Cytometryg reporter gene systems is fundamental for pharmaceutical, biomedical, and molecular biology research. Genetic reporters are used not only for measuring promoter activities but also for understanding the mechanisms controlling gene transcription and in the?identification, and characterization of .-a

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