Titlebook: Mammalian Synthetic Promoters; David Gould Book 2017 Springer Science+Business Media LLC 2017 synthetic promoters.gene therapy.pharmacolog

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The Tetracycline Responsive System therapy. We have utilized pharmacologically regulated gene expression systems to achieve fine control of gene expression levels which facilitate research in basic biology and translates to use in experimental gene therapy studies. In this chapter, we outline the application of a tightly controlled tetracycline responsive gene expression system.

入會

Light-Responsive Promotersl control of gene expression in a so far unprecedented manner..In this chapter, we first describe the available light-inducible gene expression systems compatible with mammalian cells and explain their underlying mechanisms. Afterward, we give a detailed protocol for the implementation of a UVB light-inducible expression system in mammalian cells.

subordinate

Promoter Activation with Electromagnetismield response elements. Here, we describe how to examine promoter activation with extremely low-frequency electromagnetic fields, and we provide a step-by-step guide to the assembly of a solenoid suitable for promoter activation.

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潔凈

Book 2017nthetic promoters with examples of their application. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and

Acclaim

978-1-4939-8421-3Springer Science+Business Media LLC 2017

范例

Mammalian Synthetic Promoters978-1-4939-7223-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

Intact

David GouldIncludes cutting-edge methods protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts