Titlebook: Mammalian Cell Engineering; Methods and Protocol Ryosuke Kojima Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 202

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Green Light-Controlled Gene Switch for Mammalian and Plant CellsNA operator sequence CarO. The switch is characterized by high reversibility, high transgene expression levels, and low leakiness, leading to up to 350-fold induction ratios in mammalian cells. In this chapter, we describe the essential steps to build functional components of the green light-regulat

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Sonogenetic Modulation of Cellular Activities in Mammalian Cellsfically manipulate activities in genetically defined cells but not in unmodified cells present in the ultrasound-illuminated regions. These approaches provide new strategies for noninvasive modulation of target cells in various therapeutic applications.

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Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diarams, but also by a custom-engineered Bluetooth-active glucometer in a semiautomatic and glycemia-dependent manner. As a result, blood glucose homeostasis was semiautomatically maintained in diabetic mice through the smartphone-controlled semiautomatic theranostic system. By combining digital signal

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Multiplexed Genome Engineering with Cas12arams. In this chapter, we outline the utility of SiT-Cas12a in human cells and describe experimental procedures for executing massively multiplexed genome engineering experiments-including strategies for designing and assembling customized multiplexed CRISPR guide RNA arrays as well as validating an

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Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPTsed SLIPT system for inducing (1) reversible protein translocation and (2) synthetic activation of the Raf/ERK pathway. This system provides a simple and versatile tool in mammalian synthetic biology for temporally manipulating various signaling molecules and pathways at the PM.