Titlebook: Mammalian Cell Engineering; Methods and Protocol Ryosuke Kojima Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 202

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Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cellsfter . Cas9 was first demonstrated for mammalian genome editing, many CRISPR-associated (Cas) protein variants have been isolated from different species and adopted for genome editing. Furthermore, various effector domains have been fused to these Cas proteins to expand their genome-editing abilitie

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Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPTtranslocation (SLIPT) is an emerging platform that enables control of protein localization in living mammalian cells using synthetic self-localizing ligands (SLs). We recently established a chemogenetic SLIPT system, in which any protein of interest fused to an engineered variant of . dihydrofolate

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prising to see that 85% of these injuries are inversion distorsion traumata of the ankle (Nicholas and Hershman 1986). Ankle sprains account for 3%–10% of all emergency department visits (Brostrom 1965; Hume 1984; Muwanga 1986). The ankle joint is most vulnerable in sports such as volleyball, basket

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Leo Schellerl information that can be provided in a unique, non-invasive way by nuclear magnetic resonance spectroscopy (MRS). Yet, the basic principles of MRI and MRS are quite similar. Nuclear magnetic resonance relies on the response of atomic nuclei, which are polarized by a strong magnetic field, to a radi

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