Titlebook: Maize; Methods and Protocol L. Mark Lagrimini Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Corn yield.Plant

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Plant Gene Regulation Using Multiplex CRISPR-dCas9 Artificial Transcription Factorso independent miRNA genes simultaneously in Arabidopsis. We then describe a “higher-order” vector assembly method for increased multiplexing capacity. This higher-order assembly method in principle allows swift stacking of gRNAs cassettes that are only limited by the loading capacity of a cloning or expression vector.