Titlebook: Lymphangiogenesis; Methods and Protocol Guillermo Oliver,Mark L. Kahn Book 2018 Springer Science+Business Media, LLC, part of Springer Natu

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Imaging of Endothelial Cell Dynamic Behavior in Zebrafish, and cellular events shaping the embryonic vasculature. In this chapter, we aim to present the methods for the measurement of some of the most commonly investigated dynamic parameters in endothelial cells during developmental angiogenesis, namely, migration speed and acceleration, filopodia extensio

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Investigating Effects of Fluid Shear Stress on Lymphatic Endothelial Cells,ascular development, including vascular remodeling, to create a hierarchical collecting vessel network and lymphatic valves (Sweet et al., J Clin Invest 125, 2995–3007, 2015). However, mechanistic insights into the response of lymphatic endothelial cells to fluid flow are difficult to obtain from in

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Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo,uscle physiology has remained elusive. With the advent of genetic methods to manipulate gene expression in mice, a set of new tools became available for the investigation and visualization of the lymphatic vasculature. To gain insight into the molecular regulators of lymphatic contractile function,

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Evaluation and Characterization of Endothelial Cell Invasion and Sprouting Behavior,esented whereby ECs are induced to sprout from a monolayer surface or from aggregated ECs suspended within a collagen gel matrix. In each case, the assays are performed using serum-free defined media containing a combination of five growth factors (Factors): FGF-2, SCF, IL-3, SDF-1α, and insulin. In

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Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis,ich lymphangiogenesis can be observed and manipulated. This model has many advantages, including access for intravital immunostaining and imaging to assess morphological features and regeneration kinetics, as well as functional assays such as lymphatic clearance. We describe five procedures: (1) the