Titlebook: Lymphangiogenesis; Methods and Protocol Guillermo Oliver,Mark L. Kahn Book 2018 Springer Science+Business Media, LLC, part of Springer Natu

過渡時(shí)期

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moribund

Characterization of Zebrafish Facial Lymphatics,specific roles for molecules implicated in lymphatic development. This chapter provides protocols for visualization and analysis of facial lymphatic development in the zebrafish and may be applied in developmental or drug discovery studies.

Hyperplasia

Morphological and Functional Analysis of CNS-Associated Lymphatics,ological techniques involving a combination of histological, flow-cytometric, and functional drainage assays are applied to brain and spinal cord meninges and detailed stepwise procedures used for successful in vivo and ex vivo characterization of meningeal lymphatic vessels.

萬花筒

Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo, large animals, and later adapted to rat vessels, here we describe modifications that enable the study of isolated, pressurized murine lymphatic collecting vessels. These methods, used in combination with transgenic mice, can be a powerful tool to investigate the molecular and cellular mechanisms of lymphatic function.

locus-ceruleus

鉗子

Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development,nction. Here we describe a protocol that combines the strengths of fluorescence microscopy and scanning electron microscopy to precisely locate and analyze the topography of developing lymphovenous valves at high resolution.

逃避現(xiàn)實(shí)

,Morphological Analysis of Schlemm’s Canal in Mice,raocular pressure and is sensitive to defects in blood and lymphatic vascular signaling pathways. Here, we describe a method to stain and quantify SC area and morphology in enucleated mouse eyes, providing a tool for understanding its development and function in small animal genetic or disease models.

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