Titlebook: Long Non-Coding RNAs; Methods and Protocol Yi Feng,Lin Zhang Book 2016 Springer Science+Business Media New York 2016 long non-coding RNAs (

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,Simultaneous RNA–DNA FISH,cellular contexts. However, a simple combination of RNA FISH and DNA FISH often generates disappointing results because the fragile RNA signals are often damaged by the harsh conditions used in DNA FISH for denaturing the DNA. Here, we describe a robust and simple RNA–DNA FISH protocol, in which ami

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Visualizing Long Noncoding RNAs on Chromatin, information on both the expression level and localization of RNA transcripts and, when combined with detection of associated proteins and chromatin modifications, can lend essential insights into long noncoding RNA (lncRNA) function. Epigenetic effects have been postulated for many lncRNAs, but sho

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Non-isotopic Method for , LncRNA Visualization and Quantitation,As (lncRNAs). Genome-wide studies have identified thousands of lncRNAs lacking protein-coding capacity. RNA in situ hybridization technique is especially beneficial for the visualization of RNA (mRNA and lncRNA) expression in a heterogeneous population of cells/tissues; however its utility has been

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Detection of Long Noncoding RNA Expression by Nonradioactive Northern Blots,his finding prompted intensive investigations on the biological functions of noncoding RNAs and led to very exciting discoveries of microRNAs as important players in disease pathogenesis and therapeutic applications. Research on long noncoding RNAs (lncRNAs) is in its infancy, yet a broad spectrum o

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Methods for Characterization of Alternative RNA Splicing,R using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of particular splice isoforms. We also describe analysis of alternative splicing using a splicing “minigene” in mammalian cell tissue culture to facilit

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