Titlebook: Live Cell Imaging; Methods and Protocol Dmitri B. Papkovsky Book 2010 Humana Press 2010 Data analysis algorithms.Multi-mode microscopes.Mul

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Two-Photon Permeabilization and Calcium Measurements in Cellular Organellesatic acinar cells, this technique allowed us to show that all three messengers – IP., cADPR, and NAADP – release Ca. from two intracellular stores: the endoplasmic reticulum and an acidic store in the granular region. This chapter describes a detailed procedure of using this technique with pancreatic acinar cells.

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Analysis of Mitochondrial pH and Ion Concentrationsring buffer and calibration solutions, extensive annotated protocols are provided. In addition, detailed measurement and image analysis protocols are given to routinely obtain optimum results with confidence, while avoiding a number of typical pitfalls.

Infant

Labels and Probes for Live Cell Imaging: Overview and Selection Guideables that can impact the utility of these fluorescent materials for imaging, selection of the appropriate probes can be a difficult task. In this report an overview of fluorescent imaging agents and details on their optical and physical properties that can impact their function are presented.

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Book 2010ular biology technique to become even more powerful, versatile, and affordable. In .Live Cell Imaging: Methods and Protocols., a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by repres

Microgram

The Application of Fluorescent Probes for the Analysis of Lipid Dynamics During Phagocytosised by laser scanning or spinning disc confocal microscopy. While the focus of this chapter is on phagocytic events, this general method can be employed for the analysis of lipid distribution and dynamics during a variety of biological processes in the cell type of the investigator’s choice.

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