Titlebook: Lipidomics; Methods and Protocol Sanjoy K. Bhattacharya Book 2017 Springer Science+Business Media LLC 2017 biomolecules.signaling.mass spec

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Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells,e and chemoresistance in pancreatic cancer. In spite of its role as a stem cell marker in pancreatic cancer, its function remains elusive. CD133 (also known as prominin-1) is a pentaspan glycoprotein predominantly localized in lipid rafts, specialized membrane microdomains enriched in crucial signal

surrogate

Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation,seful for detecting fatty acids and lipid molecules that are targeted to specialized membranes. Without fractionation, these types of molecules could be below the levels of detection after being diluted out by the more abundant lipid molecules with a more ubiquitous distribution throughout the vario

companion

Adrenaline

Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLCerol content of cells is often challenging. Traditional methods use enzymatic assays in which an indirect measurement of the esterified cholesterol content is obtained by subtracting the measurements of the free from the total cholesterol content. However, this approach fails in the case where the t

IVORY

High-Performance Chromatographic Separation of Cerebrosides,, we describe the analytical separation of glucosylceramide and galactosylceramide by HPTLC. This technique can be used for quantitation purposes but also with small modification for subsequent mass spectrum analyses for structural determination.

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Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid1–139, 2015). From a mass spectrometric point of view, by lipidomics we understand targeted or untargeted mass spectrometric analysis of lipids using either liquid chromatography (LC) (Castro-Perez et al., J Proteome Res 9(5):2377–2389, 2010) or shotgun (Han and Gross, Mass Spectrom Rev 24(3):367–41

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Perineum

A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue Using Liquid-Chromatography Hiversity of lipids requires a tailored lipidomics workflow for each sample type. Therefore, every protocol in the lipidomics workflow, especially those involving sample preparation, should be optimized to avoid the introduction of bias. The coupling of ultra-high-performance liquid chromatography (UH

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