Titlebook: Legionella; Methods and Protocol Carmen Buchrieser,Hubert Hilbi Book 2019Latest edition Springer Science+Business Media, LLC, part of Sprin

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Fermentation

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Scar-Free Genome Editing in a suicide cassette strategy, this genetic toolbox enables to generate clean scar-free deletions, single-nucleotide mutation, transcriptional or translational fusions, as well as insertion at any chosen place in . chromosome, therefore enabling multiple mutations with no need of multiple selection markers.

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