Titlebook: Legionella; Methods and Protocol Carmen Buchrieser,Hubert Hilbi Book 2019Latest edition Springer Science+Business Media, LLC, part of Sprin

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Single Cell Analysis of , and ,-Infected , by Agarose Embedmentractions are frequently conducted using clonal bacterial populations and population level analysis, overlooking the remarkable differences in single cell behavior. The fastidious nutrient requirements of extracellular . and the extraordinary motility of . hamper an analysis at single cell resolution

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Mitochondrial Dynamics and Activity in ,-Infected Cellsmitochondrial dynamics and activity in living cells. Recently, new, powerful techniques such as high-content microscopy or mitochondrial respiration assays (.) have been developed to quantitatively analyze mitochondrial parameters. Here we present state-of-the-art methods adapted to analyze mitochon

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Perturbation of , Cell Infection by RNA InterferenceA interference (RNAi) has proven to be a robust and economic technique to study .-host cell interactions. Predesigned and often validated double-stranded (ds) RNA oligonucleotides that silence specific genes are commercially available. RNAi results in a reduced level of distinct proteins, which allo

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Isolation of the Dot/Icm Type IV Secretion System Core Complex from e here the procedure to isolate the core complex of the Dot/Icm type IV secretion system of . based on detergent lysis of bacteria and ultracentrifugation. The isolated protein complex can be applied for biochemical and transmission electron microscopy analysis.

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Carmen Buchrieser,Hubert HilbiIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/583814.jpg

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The Pathometabolism of , Studied by Isotopologue Profiling of the isotopologue compositions in amino acids or other metabolites, the incorporation rates of the supplied precursors and the pathways of their utilization can be studied in considerable detail. In this chapter, the method of isotopologue profiling is illustrated with recent work on the metabolism of intracellular living ..

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