Titlebook: Lactic Acid Bacteria; Methods and Protocol Makoto Kanauchi Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under e

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Tomoyuki Hibi,Hideki Kinoshita This approach helps participants connect with their true identity, expand their consciousness, and transform their personal and management development. It is an invitation to awaken the hero within, making a challenging journey of self-leadership and consciousness. Starting from the hero’s journey,

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Introduction of Spontaneous Mutations Using Streptomycin as a Method for Lactic Acid Bacteria Breediin bacterial breeding. In contrast to UV-based mutagenesis, this method allows control of the mutation sites, specifically targeting the . gene. The outlined methodology introduces spontaneous mutations using streptomycin in . GG ATCC 53103 (LGG), a widely studied lactic acid bacterium. Streptomycin

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The Chromosomal Gene Manipulation Method for , subsp. , Using a Conjugative Shuttle Vector pGMβ1re have been few reports of gene deletion, insertion, or replacement. We have developed a system that enables chromosomal gene manipulation of . using a conjugal transfer vector and easily vector construction in .. As an example, we have deleted a regulatory gene for the extracellular polysaccharide

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Preparation and Structural Analysis of Lipoteichoic Acid on Cell Membranes Derived from Lactic Acid cally composed of hydrophilic glycerolphosphate polymer and hydrophobic anchor glycolipid moieties. It is involved in physiological properties of the cell surface and also plays roles in interactions with the host. Appropriate preparation procedures, such as extraction and purification, are required

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Applications of the Third-Generation DNA Sequencing Technology to the Identification of Spoilage Miciversal primers to identify virtually all the bacterial or yeast/fungal species via the amplification and nucleotide sequencing of common ribosomal DNA regions. The simplicity of its protocol makes the method suitable for both small and large breweries.

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Isolation of Lactic Acid Bacteria Eliminating Trimethylamine (TMA) for Application to Fishery Proces during storage. It is produced from trimethylamine oxide (TMAO), an osmosis-regulating substance in fish cells that functions by a reduction reaction. Bacterial growth in fish meat increases TMA. Its odor reduces the commercial value of the meat. Technologies for its regulation and elimination are