Titlebook: Label-Free Super-Resolution Microscopy; Vasily Astratov Book 2019 Springer Nature Switzerland AG 2019 Label-free Imaging.Optical Nanoscopy

暗語

Tomographic Diffractive Microscopy: Principles, Implementations, and Applications in Biology,scopy (DHM), which delivers 3D quantitative images of the index of refraction distribution within the observed sample. It is a two-step imaging approach based first on recording of multiple holograms under varying conditions of illumination, and second on applying sample-adapted numerical inversion

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Absorption-Based Far-Field Label-Free Super-Resolution Microscopy,nt methods for extensive non-fluorescent species. In this chapter, we review recent achievements of far-field label-free super-resolution microscopy (LFSRM) that deploys materials absorption to provide the contrast. In the linear absorption modalities, samples convert photon energy to heat efficient

sundowning

,Label-Free Pump–Probe Nanoscopy,f advanced super-resolution fluorescence microscopy techniques to circumvent the diffraction limit. Despite their well-established benefits, these techniques have to rely on the photo-physical properties of fluorescent molecules to obtain the desired contrast and spatial resolution. The labeling pro

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挑剔為人

招致

Label-Free Super-Resolution Microscopy by Nonlinear Photo-modulated Reflectivity,lectance of materials, induced by an ultra-short pump pulse. In NPMR, a modulated train of pump pulse is focused on the sample that photo-excites temperature and/or charge-carriers changes, spatially distributed inside the diffraction-limited spot. A spatially overlapping, delayed, and unmodulated t

訓(xùn)誡

Nonlinear Label-Free Super-Resolution Microscopy Using Structured Illumination,ound 200 nm restricting its applicability. However, recent advances in optical super-resolution techniques have shown that the diffraction?does not impose a fundamental limit to resolution and can be circumvented. These super-resolution?techniques can provide resolution approaching the nanometer?sca

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