Titlebook: Knowledge Mining; Proceedings of the N Spiros Sirmakessis Conference proceedings 2005 Springer-Verlag Berlin Heidelberg 2005 Document Proce

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Furio Camillo,Melissa Tosi,Tiziana Traldirmation. Here, using the 10× Genomic platform and the most widely cited bioinformatics tools, we describe how to analyze early female germ cell development using scRNA-seq data generated from mouse E11.5 to E14.5 ovaries. This pipeline will provide a guide for exploring the processes of early germ c

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Dimitrios Mavroeidis,George Tsatsaronis,Michalis Vazirgiannisedure for PGCLC differentiation with the balanced induction of SOX17 and PRDM1. The protocol can be used for PGC induction in other mammalian species exhibiting PGCs with SOX17 expression. Together, these studies will advance the understanding of germ cell biology and its applications in reproductiv

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Georgia Panagopoulouedure for PGCLC differentiation with the balanced induction of SOX17 and PRDM1. The protocol can be used for PGC induction in other mammalian species exhibiting PGCs with SOX17 expression. Together, these studies will advance the understanding of germ cell biology and its applications in reproductiv

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Federico Neri,Remo Raffaelliell fate to the meiotic development fate. Whether a germ cell self-renews or enters meiotic prophase is largely determined by its proximity to the distal tip cell (DTC), which is the somatic niche cell that caps the distal end of the gonad. Germ cells close to the DTC have high levels of GLP-1 Notch

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Thierry Poibeau a cellular level, the responses include cortical cytoskeletal rearrangement, nuclear envelope breakdown, assembly of the acentriolar meiotic spindle, chromosome segregation, and likely changes important for fertilization and the oocyte-to-embryo transition. This chapter focuses on signaling mechani

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depreciate

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Alf Fyhrlund,Bert Fridlund,Bo Sundgren.). However, when measured as development to term as a proportion of oocytes used, the efficiency of somatic cell NT, has been very low (1–2%). Several variables influence the ability to reproduce a specific genotype by cloning. These include species, source of recipient ova, cell type of nuclei don

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Martin Rajman,Martin Vesely understanding of basic developmental biology, but also has critical implications in animal husbandry and biotechnology (.), as well as in assisted reproductive medicine (.). Experimental manipulation of the cytoplasm of oocytes and early embryos has been a significant component of this research. Al

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