Titlebook: Kinase Screening and Profiling; Methods and Protocol Hicham Zegzouti,Said A. Goueli Book 2016 Springer Science+Business Media New York 2016

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書目名稱Kinase Screening and Profiling影響因子(影響力)




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書目名稱Kinase Screening and Profiling網絡公開度學科排名




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書目名稱Kinase Screening and Profiling被引頻次學科排名




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書目名稱Kinase Screening and Profiling年度引用學科排名




書目名稱Kinase Screening and Profiling讀者反饋




書目名稱Kinase Screening and Profiling讀者反饋學科排名

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Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy,lar interaction between the protein and the ligand, and has many advantages in fragment screening over other biophysical techniques. This chapter describes the practical aspects of fragment screening by saturation transfer difference NMR.

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Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells,visualizing the protein phosphorylation in living cells. Using these indicators, we visualized under a fluorescence microscope when, where, and how the protein kinases are activated in single living cells.

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周年紀念日

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Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Formacan be coupled to FLuc through a two-step process. This chapter describes the bioluminescent assays that were developed for three classes of kinases (lipid, protein, and metabolic kinases) and miniaturized to 1536-well format, enabling their use for quantitative high-throughput (qHTS) of small-molecule libraries.

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A High-Content Assay to Screen for Modulators of EGFR Function,on are imaged and quantified using time-dependent granule formation in cells. This method can readily be used to search for EGFR modulators in both chemical and RNAi screening; with potential applicability to other receptor tyrosine kinases.

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