Titlebook: KRAS; Methods and Protocol Andrew G. Stephen,Dominic Esposito Book 2024 Leios Biomedical Research, Inc. 2024 GTPase activity.mass spectrome

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Luminescence-Based Techniques for KRAS Thermal Stability Monitoring,uminescence detection in its varying forms. Among these methods, thermal stability assays, using luminophore-conjugated proteins or external environment sensing dyes, are widely used. In this chapter, we describe methods enabling KRAS stability monitoring in vitro, with an emphasis on ligand-induced

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Analysis of the Guanine Nucleotide-Bound State of KRAS by Ion-Pair Reversed-Phase High-Performance (IP-RP-HPLC)-based method, which enables analyzing GDP and GTP bound to small GTPases immunoprecipitated from cells. The activation status of FLAG-KRAS expressed in HEK293T cells can be investigated with the IP-RP-HPLC method. This method also can be adapted to determine the effects of compounds suc

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