Titlebook: Investigation of Nanoscopic Dynamics and Potentials by Interferometric Scattering Microscopy; Jaime Ortega Arroyo Book 2018 Springer Inter

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Structural Dynamics of Myosin 5a,This chapter provides the results obtained from single-molecule studies using iSCAT on a construct of myosin 5a with an N-terminal biotin ligand, which was conjugated with different sized gold nanoparticles, and a C-terminal GFP ligand.

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All Optical Label-Free Detection, Imaging and Tracking of Single Proteins,This chapter details a proof of principle study with the molecular motor myosin 5a heavy meromyosin, which demonstrates that interferometric scattering microscopy can be used as a truly label-free all-optical bio-sensing platform operating at the single-molecule level.

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2190-5053 s into fundamental questions concerning molecular motors, me.This thesis offers a unique guide to the development and application of ultrasensitive optical microscopy based on light scattering. Divided into eight chapters, it covers an impressive range of scientific fields, from basic optical physic

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Book 2018cially the detailed information provided on how to design, build and implement an interferometric scattering microscope, as well as the descriptions of all instrumentation, hardware interfacing and image processing necessary to achieve the highest levels of performance, will be of interest to researchers now entering the field..