Titlebook: Invariant Natural Killer T-Cells; Methods and Protocol Chaohong Liu Book 2021 The Editor(s) (if applicable) and The Author(s), under exclus

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The Expansion and Cytotoxicity Detection of Human iNKT Cells,ntigens presented by CD1d molecules and can be specifically activated by alpha-galactosylceramide (α-GalCer) in vitro. After activation, iNKT cells expand efficiently and exert direct killing effects. Based on it, we mainly introduce the protocols of detection of human iNKT cell functions in vitro,

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Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood,tegy for the treatment of malignant and nonmalignant diseases. However, their paucity in human blood limits their immunotherapeutic applications. Herein we describe a rigorously optimized 21-day ex vivo expansion method to achieve log-fold increases in immunotherapeutic human iNKT cells.

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Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6- intravital microscopy has been widely used for real-time observation and quantification of cell movements in living animals. Here we describe the use of an in vivo half-dissociated preparation method combined with intravital confocal microscopy to observe the dynamic activities of invariant natural

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ENDOW

,Redirecting iNKT Cell Antitumor Immunity with α-GalCer/CD1d-scFv Fusion Proteins,lay key roles in cancer immunotherapy. However, administration of iNKT cell agonist αGalCer fails to induce sustained antitumor immunity due to the rapid anergy induction after an initial strong activation. To this end, we have designed a recombinant CD1d protein that is fused to an anti-TAA scFv, w

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Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles During Development,elopment. Here, we discuss several?complementary approaches, including flow cytometry, metabolic flux, and transcriptome analyses, to characterize the dynamic changes in metabolic profiles associated with invariant natural killer T cell development.

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Dapeng Zhou,Chengfeng Xia,Peng G. Wang,Zhiyuan Li,Wenpeng Zhang,Guanghui Ni,Jiaxi Chengd zur Umwelt besch?ftigen. So unterschiedlich die Autoren sind, so unterschiedlich sind auch die Denkans?tze in den Arbeiten. Einig sind sich die Autoren darin, da? Energie, Wirtschaft und Umwelt in ihrer engen Verflechtung und Wechselwirkung betrachtet werden müssen. Sie sind davon überzeugt, da? E

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Ke Wang,Rong Jin,Qing Ged zur Umwelt besch?ftigen. So unterschiedlich die Autoren sind, so unterschiedlich sind auch die Denkans?tze in den Arbeiten. Einig sind sich die Autoren darin, da? Energie, Wirtschaft und Umwelt in ihrer engen Verflechtung und Wechselwirkung betrachtet werden müssen. Sie sind davon überzeugt, da? E

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