Titlebook: Intracellular Pathogens; Methods and Protocol Aneesh Thakur Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive lice

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Median Tissue Culture Infectious Dose 50 (TCID50) Assay to Determine Infectivity of Cytopathic Virung viruses. Here, we describe the discontinuous median tissue culture infectious dose 50 (TCID.) assay to quantitatively determine the titer of any virus that can produce a visible cytopathic effect in infected cells.

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Phagocytosis,ling to activate the internalization machinery, (iii) phagosome formation, and (iv) phagolysosome maturation. In this chapter, we present a general view of our current knowledge on phagocytosis performed mainly by professional phagocytes through antibody and complement receptors and discuss aspects that remain incompletely understood.

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Quantitative Proteomics of the Intracellular Bacterial Pathogen , Serovar Typhimurium,ncluding protein extraction, digestion, and purification, as well as mass spectrometry measurements and bioinformatics analysis. The data generated from our dual perspective profiling approach provides new insight into pathogen and host protein modulation under infection-like conditions.

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Production, Isolation, and Detection of Poly IC in Extracellular Vesicles from U937 Cells,an anti-dsRNA antibody (J2) for detection. Two methods for isolating the EVs and two methods for isolating the RNA from these EVs are described. Together, these methods effectively produce, isolate, and detect long dsRNA from EVs.

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In Situ Characterization of Human Follicular Helper CD4 T Cells,ity, understanding the cellular and molecular mechanisms regulating Tfh cell development is of great importance. Here, we describe novel approaches for the comprehensive understanding of these cells and possible implications for future studies in vaccine development and the understanding of the pathogenesis of relevant diseases.

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Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR, studies. In this chapter, we describe how .H NMR can be combined with a cellular infection model to study the metabolic crosstalk between a bacterial pathogen and its host both in the extracellular and intracellular compartments. Central carbon metabolism is highlighted by using glucose labeled with the stable isotope .C.