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Titlebook: Insoluble Proteins; Methods and Protocol Elena García-Fruitós Book 2015 Springer Science+Business Media New York 2015 Biotechnology.Express

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發(fā)表于 2025-3-21 18:32:01 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Insoluble Proteins
副標(biāo)題Methods and Protocol
編輯Elena García-Fruitós
視頻videohttp://file.papertrans.cn/468/467852/467852.mp4
概述Includes cutting-edge methods and protocols involving insoluble proteins.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.I
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Insoluble Proteins; Methods and Protocol Elena García-Fruitós Book 2015 Springer Science+Business Media New York 2015 Biotechnology.Express
描述.With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. .Insoluble Proteins: Methods and Protocols. includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and practical, .Insoluble Proteins: Methods and Protoc
出版日期Book 2015
關(guān)鍵詞Biotechnology; Expression systems; Insolubility; Pharmaceuticals; Protein production; Protein purificatio
版次1
doihttps://doi.org/10.1007/978-1-4939-2205-5
isbn_softcover978-1-4939-4675-4
isbn_ebook978-1-4939-2205-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
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書目名稱Insoluble Proteins網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




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書目名稱Insoluble Proteins被引頻次學(xué)科排名




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978-1-4939-4675-4Springer Science+Business Media New York 2015
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Transient Expression in HEK 293 Cells: An Alternative to , for the Production of Secreted and IntracTransient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.
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General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins,lic bacteria, are gaining interest in this field. However, it is worth mentioning that prokaryotic expression system poses, in many cases, severe restrictions for a successful heterologous protein production. Thus, eukaryotic systems such as mammalian cells, insect cells, yeast, filamentous fungus,
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Co-translational Stabilization of Insoluble Proteins in Cell-Free Expression Systemsworking concentrations. Examples of screening experiments in order to systematically identify compounds or compound mixtures that stabilize particular proteins of interest are given. The method can primarily be considered for the production of unstable soluble proteins or of membrane proteins contai
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Book 2015ies format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Comprehensive and practical, .Insoluble Proteins: Methods and Protoc
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