Titlebook: In Situ Hybridization Protocols; Boye Schnack Nielsen Book 2014Latest edition Springer Science+Business Media New York 2014 RNA.RNA molecu

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978-1-4939-5526-8Springer Science+Business Media New York 2014

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Fixation/Permeabilization Procedure for mRNA In Situ Hybridization of Zebrafish Whole-Mount Oocyteshe simultaneous fixation/permeabilization of samples using formaldehyde as fixative and short C-chain aliphatic carboxylic acids, particularly glacial acetic acid, as permeabilizers. As compared with in situ hybridization performed with routine methods, our procedure is simpler and provides better s

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Whole-Mount In Situ Hybridization Using DIG-Labeled Probes in Planarian,ybridization (WISH) and fluorescent in situ hybridization (FISH) are key and most commonly used techniques to determine and visualize gene expression patterns in planaria. Here, we present the established version of whole-mount in situ hybridization (WISH) and whole-mount fluorescence in situ hybrid

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LNA-Based In Situ Hybridization Detection of mRNAs in Embryos,perimental manipulations. ISH using short DNA probes containing locked nucleic acid nucleotides (LNAs) holds the additional advantage of allowing the detection of specific RNA splice variants or of closely related family members that differ in only short regions, creating new diagnostic and detectio

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,Chromogen Detection of microRNA in Frozen Clinical Tissue Samples Using LNA? Probe Technology,sections has been facilitated by locked nucleic acid (LNA)-based probe technology and can be performed within a single working day. In the current method paper, we present a similar simple 1-day ISH method developed for cryostat sections obtained from clinical cryo-embedded tissue samples. The prese