Titlebook: In Situ Hybridization Methods; Giselbert Hauptmann Book 2015 Springer Science+Business Media New York 2015 TransISH.chromogenic RNA ISH te

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Detection of mRNA and microRNA Expression in Basal Chordates, Amphioxus and Ascidiansnclusion, the method described in this chapter permits the detailed visualization of gene expression at single-cell resolution and makes it a useful tool for analyzing expression patterns of neuron specific genes in both amphioxus and ascidians.

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Gina Broitman-Maduro,Morris F. Maduroations for the communication of science in general and will be of interest to a wide audience, including space scientists, science communication professionals, people fascinated by exploration and discovery, st978-3-030-33214-3978-3-030-33212-9Series ISSN 2199-3882 Series E-ISSN 2199-3890

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David D. R. Brown,Bret J. Pearsonons and researchers, including counsellors, psychotherapists, family therapists, psychiatrists, nurses, play therapists, speech and language therapists, and mental health social workers..978-3-031-30417-0978-3-031-30418-7Series ISSN 2662-9127 Series E-ISSN 2662-9135

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0893-2336 that ensures successful results in the laboratory..Essential and authoritative, .In Situ Hybridization Methods?.provides detailed protocols for newcomers to ISH,?and inspires rese978-1-4939-4463-7978-1-4939-2303-8Series ISSN 0893-2336 Series E-ISSN 1940-6045

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Multicolored Visualization of Transcript Distributions in Drosophila Embryosactions that permit to highlight each mRNA expression pattern by a differing and contrasting cellular color precipitate. We provide tips and tricks for each critical step helpful for successful application of the tricolor whole-mount in situ hybridization (WISH) method.

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Large-Scale ISH on Mouse Brain Sections for Systematic Gene Expression Analysis in Developing Mouse er understanding of the development, connectivity, and function of diencephalic neurons. Here we will describe a protocol that we have previously used to conduct large-scale, high-quality two-color ISH to characterize gene expression patterns in the developing mouse diencephalon.

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High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond a genomic level to cover organs or even whole organisms in great detail. Moreover, we discuss suitable HT instrumentation and address postproduction issues typically arising with HT pipelines such as annotation of expression data and database organization.

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One FISH, dFISH, Three FISH: Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Fthe analysis of multiple RNAs, simultaneously. Thus, considerable effort by the entire planarian community has been put forth to create adequate methodologies towards this goal. Here we summarize the field, methodological evolution, and describe a new method for more rapid and more sensitive detection of multiple RNAs by multi-fluorescent ISH.