Titlebook: In Situ Detection of DNA Damage; Methods and Protocol Vladimir V. Didenko Book 20021st edition Springer Science+Business Media New York 200

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Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assaysl science. A variety of experimental techniques including alkaline sucrose gradient centrifugation, alkaline elution, nucleoid sedimentation, viscoelastic measurements of DNA, alkaline unwinding, and gel electrophoresis (.–.) have been developed to detect DNA damage in large cell populations. Howeve

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1064-3745 Sometimes the “in situ” term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluoresc978-1-61737-272-8978-1-59259-179-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Quantitative Differentiation of Both Free 3′ OH and 5′ OH DNA Ends Using Terminal Transferase-Based .,.). Recently, Sahara et al. reported the discovery of a caspase-3-activated protein that is required for apoptotic chromatin condensation without DNA fragmentation (.). Therefore, chromatin condensation and DNA fragmentation are independent events of apoptosis. Both agarose gel electrophoresis usi