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Titlebook: Immunoproteomics; Methods and Protocol Kelly M. Fulton,Susan M. Twine Book 2019Latest edition Springer Science+Business Media, LLC, part of

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發(fā)表于 2025-3-21 20:02:38 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Immunoproteomics
副標(biāo)題Methods and Protocol
編輯Kelly M. Fulton,Susan M. Twine
視頻videohttp://file.papertrans.cn/463/462279/462279.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Immunoproteomics; Methods and Protocol Kelly M. Fulton,Susan M. Twine Book 2019Latest edition Springer Science+Business Media, LLC, part of
描述This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?.Cutting-edge and thorough, .Immunoprotemics: Methods and Protocols, Second Edition. is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhancedhands-on insights into this evolving field.?.
出版日期Book 2019Latest edition
關(guān)鍵詞MHC peptides; Immunogenicity; autoimmunity; Glycopeptides; SERPA; Proteomics
版次2
doihttps://doi.org/10.1007/978-1-4939-9597-4
isbn_softcover978-1-4939-9599-8
isbn_ebook978-1-4939-9597-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

書目名稱Immunoproteomics影響因子(影響力)




書目名稱Immunoproteomics影響因子(影響力)學(xué)科排名




書目名稱Immunoproteomics網(wǎng)絡(luò)公開度




書目名稱Immunoproteomics網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Immunoproteomics被引頻次




書目名稱Immunoproteomics被引頻次學(xué)科排名




書目名稱Immunoproteomics年度引用




書目名稱Immunoproteomics年度引用學(xué)科排名




書目名稱Immunoproteomics讀者反饋




書目名稱Immunoproteomics讀者反饋學(xué)科排名




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發(fā)表于 2025-3-21 22:56:28 | 只看該作者
Immunoproteomics Methods and Techniques,s or cancerous cells. The study of immune biomarkers or antigens is not new, and classical methods such as agglutination, enzyme-linked immunosorbent assay, or Western blotting have been used for many years to study the immune response to vaccination or disease. However, in many of these traditional
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發(fā)表于 2025-3-22 00:55:31 | 只看該作者
Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification,protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. Proteomic approaches, including serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SE
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,Enriching for Low-Abundance Serum Proteins Using ProteoMiner? and Protein-Level HPLC,sult of such an immune response can shed light on the mechanism of disease or serve as biomarkers. These biomarkers can be used in a diagnostic capacity or as correlates of protection following vaccination. Protein levels in the circulatory system are considered representative of the system as a who
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發(fā)表于 2025-3-22 19:59:39 | 只看該作者
,Identification of the?Antigen Content of Electroimmunoprecipitates,main specificity in reactions that are best visualized by gel electroimmunoprecipitation methods (e.g., when analyzing complex antigen mixtures). The great advantage of gel immunoprecipitation approaches is that each immunoprecipitate contains antigen in a pure form and that the precipitate is separ
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發(fā)表于 2025-3-23 00:54:33 | 只看該作者
Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (Luminescence emitted by the reporter enzyme . luciferase (Ruc) fused to an antigen of interest. The LIPS assay can be utilized as a high-throughput and sensitive serological method for profiling serum antibodies recognizing diverse antigens. This chapter provides a detailed protocol for detecting hum
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發(fā)表于 2025-3-23 03:25:53 | 只看該作者
Intact Mass Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from Serum, and downstream processing technologies. The structural diversity of therapeutic antibodies has also evolved with the development of bispecific (and multispecific) antibodies and antibody-drug conjugates. With increased structural complexities and multi-modularity, there is a need to demonstrate tha
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發(fā)表于 2025-3-23 06:15:48 | 只看該作者
Antigen Identification for Cell-Binding Antibodies Using Ligand-Directed Crosslinking and Biotin Tr we describe a protocol that uses ligand-directed crosslinking with the aminooxy-sulfhydryl-biotin (ASB) trifunctional crosslinker followed by a proteomic analysis to identify the cellular receptors for orphan ligands. We describe the synthesis of the ASB crosslinker, labelling of the ligand with AS
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