Titlebook: Immunological Tolerance; Methods and Protocol Ashleigh S. Boyd Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019

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Isolation and Characterisation of Human Adipose-Derived Stem Cells immunosuppressive functionality both in vitro and in vivo. A number of researchers have described the isolation of ASCs through the enzymatic digestion of fat samples, followed by a number of purification steps, involving centrifugation and filtration. Here, we utilize a standard isolation techniqu

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Generation of Antigen-Specific T Cells from Human Induced Pluripotent Stem Cellsogeny of hematopoiesis. The development of widely varying reprogramming methods has enabled us nowadays to obtain iPSCs even from a small number of antigen-specific T cells from patients. As these T-cell-derived iPSCs (T-iPSCs) carry TCR gene rearrangements in their genomic DNA, they are likely usef

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Isolation of Human Regulatory T Lymphocytes by Fluorescence-Activated Cell Sortingr population of T lymphocytes with suppressive capacity are defined by expression of the T cell co-receptor CD4 and the interleukin-2 receptor α-chain (CD25), combined with minimal expression of the interleukin-7 receptor α subunit (CD127). We begin by outlining the method for isolating peripheral b

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Identification and Isolation of Regulatory B Cells in Mouse and Humant to have standardized protocols for their isolation and identification. Previous work by our laboratory has shown that the immature B-cell populations in the murine spleen and human peripheral blood produce the highest levels of IL-10 on engagement of CD40, and can suppress pro-inflammatory T-cell

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In Vitro Suppression of CD4+ T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cellson of immune responses in cancer, infection, and autoimmunity. Herein, we describe a protocol for the isolation of murine and human MDSCs and the assessment of their ability to suppress CD4. T-cell responses in vitro.

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