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Titlebook: Immunohistochemistry and Immunocytochemistry; Methods and Protocol Luis Del Valle Book 2022 Springer Science+Business Media, LLC, part of S

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發(fā)表于 2025-3-21 18:46:31 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱Immunohistochemistry and Immunocytochemistry
副標(biāo)題Methods and Protocol
編輯Luis Del Valle
視頻videohttp://file.papertrans.cn/463/462155/462155.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱Methods in Molecular Biology
圖書(shū)封面Titlebook: Immunohistochemistry and Immunocytochemistry; Methods and Protocol Luis Del Valle Book 2022 Springer Science+Business Media, LLC, part of S
描述.This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the theory and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls...?.Authoritative and cutting-edge, .Immunohistochemistry and Immunofluorescence: Methods and Protocols. aims to be a useful practical guide to scientists to help further their study in this field.
出版日期Book 2022
關(guān)鍵詞Tissue Processing; antigen Retrieval; Spectral Microscopy; In situ Hybridization; Photobleaching; Flow Cy
版次1
doihttps://doi.org/10.1007/978-1-0716-1948-3
isbn_softcover978-1-0716-1950-6
isbn_ebook978-1-0716-1948-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
The information of publication is updating

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Immunohistochemistry in Historical Perspective: Knowing the Past to Understand the Present,n. It has numerous applications in medicine, particularly in cancer diagnosis. It was Albert Hewett Coons, Hugh J Creech, Norman Jones, and Ernst Berliner who conceptualized and first implemented the procedure of immunofluorescence in 1941. They used fluorescein isothiocyanate (FITC)-labelled antibo
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Antigen Retrieval and Signal Amplification,or IHC enables their long-term stability and preserves tissue morphology; however, downstream analysis of protein localization within fixed samples can be complicated by cross-links formed between proteins during formalin fixation which mask target epitopes. Antigen Retrieval (AR) is a process intro
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Probes for Fluorescent Visualization of Specific Cellular Organelles,ncluding the nucleus, endoplasmic reticulum, Golgi apparatus, lysosomes, and mitochondria. Cell biologists therefore have extensively utilized organelle specific counterstains to help identify the localization of specific proteins or other targets of interest in order to garner an understanding of e
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Double Labeling Fluorescent Immunocytochemistry, as its functioning by expanding knowledge on colocalization between its components. The potency of this method is based on findings in the areas of specific antibodies production, fluorescent labels, and microscopy. Since it merges different fields, it requires basic knowledge on all the steps that
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