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Titlebook: Immunochemical Protocols; Margaret M. Manson Book 19921st edition Humana Press 1992

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書目名稱Immunochemical Protocols
編輯Margaret M. Manson
視頻videohttp://file.papertrans.cn/463/462115/462115.mp4
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Immunochemical Protocols;  Margaret M. Manson Book 19921st edition Humana Press 1992
描述Molecular biologists can no longer afford to be experts in only one par- ticular area. They need to be equally competent at handling DNA, RNA, and proteins, moving from one area to another as required by the problem. Ad- vances in the field have been greatly helped by numerous applications of antibody-antigen interactions, so that a basic knowledge of immunology has now become useful. The emphasis of Immunochemical Protocols is not on the production of antibodies, since many people will beg or buy these, but rather on the enor- mous range of applications to which these powerful reagents can be put. For completeness, however, methods are included for the production of both polyclonal and monoclonal antibodies. Techniques that may be helpful in obtaining antibodies to elusive antigens and to synthetic peptides are also provided. Both qualitative and quantitative methods are described for local- izing, characterizing, and quantifying various antigens. These range from the use of antibodies in screening DNA libraries to their application in EUSA assays for quantifying adducts in alkylated DNA Each method is described by an author who has regularly used the tech- in his or her own labor
出版日期Book 19921st edition
版次1
doihttps://doi.org/10.1385/0896032043
isbn_ebook978-1-59259-497-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1992
The information of publication is updating

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Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins,systems are being used increasingly for expression of glycoproteins because the recombinant material is glycosylated. A number of protocols will be described here that we have used successfully with both rat (Y3 and IR983F) and mouse (SP2/0) myelomas to generate MAbs to recombinant material or peptides based on cDNA sequences.
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Immunogold Probes for Light Microscopy,nt technique, are all routinely used for immunohistochemistry. Recently, the use of colloidal gold probes as immunocytochemical markers has been described for electron immunocytochemistry (.), and these are now proving to be of use at the light microscope level.
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Immunogold Probes in Electron Microscopy,on microscopy is exactly that which damages antigens with respect to reaction with specific antibody. Cell preparation for electron microscopy is therefore a compromise between retaining sufficient antigenicity while preserving the cell ultrastructure.
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Efficient Elution of Purified Proteins from Polyvinylidene Difluoride Membranes (lmmobilon) After Tgels is immense (for example, . refs. 1–5), In spite of the numerous variations in the procedure of elution, none of the available methods is entirely satisfactory. Some of the methods are very laborious, and others lead to loss of resolution or poor recovery.
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