Titlebook: Imaging Gene Expression; Methods and Protocol Yaron Shav-Tal Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer

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Lighting Up Gene Activation in Living , Embryosdeally suited to study the establishment of gene expression patterns during development. Recent improvements in RNA labeling technologies and confocal microscopy allow?for visualizing gene activation and quantifying transcriptional dynamics in living . embryos. Here we review the available tools for

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Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysison have been identified, we still lack a basic understanding of the principles that regulate the spatiotemporal dynamics of mRNA turnover within single cells. To overcome this limitation, we have developed the TREAT biosensor, which allows for discrimination of intact reporter transcripts and stabil

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Imaging rRNA Methylation in Bacteria by MR-FISHuts. These bulk methods require the RNA from ~10. to 10. cells to be pooled to generate sufficient material for analysis. Here we describe a method—methylation-sensitive RNA in situ hybridization (MR-FISH)—that assays rRNA methylation in bacteria on a cell-by-cell basis, using methylation-sensitive

BOAST

Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single-Molecule Fluorescent In Sianotubes, which are long thin protrusions that are produced by numerous cell types and can connect cells that can be up to hundreds of microns apart. Potentially, mRNAs might also transfer via extracellular vesicles (EVs). Here we describe a method to detect transferred mRNA in cocultures of two dif

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Programmable Chromosome Painting with Oligopaintsst to conventional sources of probe, Oligopaints are computationally designed, synthesized on microarrays, and amplified by PCR. This approach allows for precise control over the sequences they target, which can range from a few kilobases to entire chromosomes with the same basic protocol. We have u

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