Titlebook: Hypoxia; Methods and Protocol Daniele M. Gilkes Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sprin

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Measurement of Metabolic Alteration in Immune Cells Under Hypoxiad expansion. Myeloid-derived suppressor cells (MDSCs) are heterogenous immature myeloid cells in the TME, which play an essential role in immune evasion by subverting T/NK cell-mediated killing. The immunosuppressive function of MDSCs is tightly regulated to the metabolic pathways, in which hypoxia

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https://doi.org/10.1007/978-3-642-99604-7processes, tissue maintenance, and the development of pathologies. Fluorescence imaging using genetically encoded reporters enables hypoxia and oxygen imaging with cellular resolution. Thereby unrestricted visualization of hypoxic cells and regions essentially relies on the availability of oxygen-in

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https://doi.org/10.1007/978-3-322-87701-7es its effect on cancer, normal processes like embryogenesis, or other pathologies such as ischemia, depend on hypoxia-regulated mechanisms. Given the degradable nature of HIF-1/2α in the presence of oxygen, defining the role of hypoxia in modeling biological processes becomes challenging when a cel