Titlebook: Hypoxia; Methods and Protocol Daniele M. Gilkes Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sprin

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A Review of Hypoxia Imaging Using 18F-Fluoromisonidazole Positron Emission Tomography). While visual assessment is fundamental, various quantitative indices such as tumor-to-muscle ratio have also been proposed. Several novel hypoxia tracers have been invented to compensate for the limitations of [.F]FMISO.

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Oxygen Assessment in Tumors In Vivo Using Phosphorescence Lifetime Imaging Microscopy Here, we present a protocol for microscopic mapping of oxygen distribution in a mouse tumor model in vivo. We demonstrate that PLIM microscopy, in combination with an Ir(III)-based probe, enables visualization of cellular-level heterogeneity of tumor oxygenation.

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Detecting Hypoxia-Inducible Factor Levels and Activityced. Accordingly, assessment of HIF-1α protein levels and HIF transcriptional activity serve as an indirect indicator of hypoxia. In this series of protocols, I describe three methods to probe the HIF pathway.

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https://doi.org/10.1007/978-3-642-99604-7 xenografted tumors. Thus, the generation of stably transfected transgenic tumor cell lines, the in vitro calibration of the genetically encoded sensors, the surgical procedures for orthotopic xenografting of tumors in mice, and workflows for the respective sample preparation and microscopy are outlined.

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https://doi.org/10.1007/978-3-658-43206-5aging) to rapidly image NQO1 activity in hypoxic and non-hypoxic cancer cells and tumors developed in live mouse xenograft models. The specificity of the dye for NQO1 in all three procedures was verified, and the methods should be useful for both in vitro and in vivo studies.