Titlebook: Hypertension; Methods and Protocol Rhian M. Touyz,Ernesto L. Schiffrin Book 2017 Springer Science+Business Media LLC 2017 hypertension.Reni

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Measurement of Angiotensin Peptides: HPLC-RIA,peptides. In order to understand the functions of the system, characterization of angiotensin peptides (products and substrates) is important. Radioimmunoassays with the requisite specificity and sensitivity have been developed to allow for the characterization and quantification of circulating and

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Dopaminergic Immunofluorescence Studies in Kidney Tissue, much of today’s research focuses on isolated nephron segments or cells from nephron segments grown in tissue culture, an often overlooked technique that can provide a unique view of many cell types in the kidney is slice culture. Here, we describe techniques that use freshly excised kidney tissue f

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Techniques for the Evaluation of the Genetic Expression, Intracellular Storage, and Secretion of Po CNP are described below. They have proven highly reliable in our hands when the steps outlined are followed as described. Given the generic nature of the procedures, these should be applicable to other polypeptides.

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Measuring T-Type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells,ility under physiological and pathophysiological conditions. The ability to enzymatically dissociate blood vessels and acutely isolate vascular smooth muscle cells has enabled the application of patch clamp electrophysiology to the identification of diverse voltage dependent ion channels that ultima

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In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and sive changes, (2) observe kinase activation, (3) manipulate signal transduction pathways, and (4) observe physiologic outputs. This chapter will provide the reader with overviews of the techniques our lab uses to inhibit signaling pathways with inhibitory RNAs and the outputs we use to monitor the e