Titlebook: Human Papillomaviruses; Methods and Protocol Clare Davy,John Doorbar Book 2006 Humana Press 2006 DNA.PCR.Southern Blot.Virus.cell lines.gen

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,Propagation of Infectious, High-Risk HPV in Organotypic “Raft” Culture,cycle. In this chapter, we describe in detail how to (1) culture keratinocytes, (2) electroporate primary keratinocytes with HPV DNA, (3) detect episomal HPV genomes by Southern (DNA) blotting, (4) grow organotypic raft cultures, (5) isolate HPV, and (6) perform in vitro infectivity testing.

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Book 2006study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze pa

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Identification of New Papillomavirus Types,f these infections to a multiplicity of clinical manifestations. The cloning and sequencing of PCR-amplified products has, to date, resulted in the identification of more than 300 putative “new” papillomavirus types. The methods used to identify these unknown papillomavirus sequences are described h

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Histochemical Analysis of Cutaneous HPV-Associated Lesions,ous human papillomavirus-associated lesions. Every step of the staining procedure, from sampling of the specimens to microscopic examination of the stained sections, is detailed with reference to the recent achievements in this field.