Titlebook: Human Monoclonal Antibodies; Methods and Protocol Michael Steinitz Book 2019Latest edition Springer Science+Business Media, LLC, part of Sp

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Antibody Fragments Humanization: Beginning with the End in Mind,to maintain antigen-binding characteristics. The best humanized candidates are identified through selection steps including in silico analysis, research scale production followed by early functional evaluation, purification assays, aggregation, and stability assessment.

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Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice,t common chromatographic mAb andals expressing human Ig genes. This chapter describes the type of transgenic-knockout mice generated for various research groups, provides examples of human mAbs developed by research groups and companies, and includes protocols of immunization, generation, production

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1064-3745 ible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Uniqueand thorough, .Human Monoclonal Antibodies: Methods and Protocols, Second Edition. is a valuable tool for novice and expert researchers interested in learning more about this evolving field..978-1-4939-8958-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Jenna J. Guthmiller,Haley L. Dugan,Karlynn E. Neu,Linda Yu-Ling Lan,Patrick C. Wilson

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