Titlebook: Human Embryonic Stem Cell Protocols; Kursad Turksen Book 2016Latest edition Springer Science+Business Media New York 2016 Derivation of ce

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Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR,t how to apply the RT-PCR technique, protocols for its performance, experimental setup, and software analysis, as of the gene expression of hESC lines in consecutive passages for long-term culture surveillance.

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Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonenewal of HESCs. We also describe a protocol and tips for conducting gene chip analysis focusing on widely used Affymetrix Microarrays. These techniques will provide us unprecedented scale of biological information that would illuminate a key to decipher complex signaling networks controlling pluripotency.

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Study of Gap Junctions in Human Embryonic Stem Cells,ommon characteristic of human embryonic stem cells (hESC) maintained in different culture conditions. This chapter introduces methods to study gap junctions in hESC, from expression of gap junction proteins to functional study of GJIC in hESC proliferation, apoptosis, colony growth, and pluripotency.

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Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate,m self-renewal of hPSCs in defined, xeno-free medium with dissociated single-cell passaging. Here, we describe a modified culture system with similar performance to efficiently expand hPSCs under defined, xeno-free conditions using a non-biological synthetic substrate.

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