Titlebook: Human Embryonic Stem Cell Protocols; Kursad Turksen Book 20061st edition Humana Press 2006 DNA.biology.cancer.cell.microarray.mitochondria

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In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells, colony culture. ES cell colonies are detached intact from the fibroblast feeder layer using dispase or collagenase. The ES cell aggregates, after 4-6 d in suspension culture, are adhered to the culture surface and form colonies of monolayer in a chemically defined medium. Under this culture conditi

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Genetic Manipulation of Human Embryonic Stem Cells by Transfection, genetic modifications while remaining pluripotent, and can be selectively propagated, allowing the clonal expansion of genetically altered cells in culture. Since the first isolation of ES cells in mice, many effective techniques have been developed for gene delivery and manipulation of ES cells. T

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Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonr of which is used to maintain or expand undifferentiated hESCs, and the latter can be used for preparation of pure hESCs RNA samples, or for screening factors influential on self-renewal of hESCs. Also described in this chapter: a protocol and tips for conducting gene chip analysis focusing on wide

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Human Embryonic Stem Cells, protocols for the potential future clinical application of hES cells. After the scientific and political obstacles are overcome, it is anticipated that the hES cell field will make a tremendous difference in conditions, such as burn traumas and diabetic foot ulcers, as well a number of degenerative

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Transplantation of Human Embryonic Stem Cells to the Chick Embryo, and integrate into the tissues of the chick embryo. Some of the hESCs differentiate and the type of embryonic microenvironment that the implanted cells were exposed to modified their differentiation. Therefore, this hESC-chick embryo system has potential for complementing studies in rodents and in