Titlebook: Human Cytomegaloviruses; Methods and Protocol Andrew D. Yurochko Book 2021Latest edition Springer Science+Business Media, LLC, part of Spri

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Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation,n and findings have been recapitulated in vivo using a humanized mouse model for HCMV latency. Therefore, this system offers a powerful approach to defining virus–host interactions and mechanisms important for HCMV latency and reactivation.

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Construction of Human Cytomegalovirus Mutants with Markerless BAC Mutagenesis,mely, recombination in infected cells occurs only in a few clones, and their selection is not possible if the targeted genes are relevant for virus replication and are not able to compete for growth against the unrecombined parental viruses. On the other hand, BAC mutagenesis enables the selection f

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Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions,, we describe the different methodologies used in our laboratory and others to analyze the interactions between HCMV glycoproteins and host cellular receptors during the entry stage of the viral life cycle.

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Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Part the cultures for cytopathic effects characteristic of this virus. Initially, such clinical isolates are usually strictly cell-associated, but continued propagation in cell culture increases the capacity of an HCMV isolate to release cell-free infectious progeny. Once cell-free infection is possible

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